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How To Make A Blood Smear. Parts of Blood Smear A peripheral blood film consists of 3 parts. The process is similar to making a normal thick film but instead of using a stick to spread the blood the edge of a glass microscope slide is used while applying firm pressure to create small scratches in the underlying slide. Spreader slide pushed across the slide in a jerky. Without delay place a spreader at an angle of 45 from the slide and move it back to make contact with the drop.
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Select the finger to puncture usually the middle or ring finger. Otherwise your smear may run right off of your horizontal slide and you will not have a nice feathered edge and your smear will be so thin you will have trouble finding enough cells. To make a short smear hold the spreader at a steeper angle and to make a longer smear hold it closer to the drop. Making a quality blood smear Although there are several techniques described for making blood smears most people use the wedge or push technique. The portion of blood film near the drop of blood. Prick the ring finger of left hand with the help of prickling needle under all aseptic conditions discard the first two drOps of blood from skin puncture site.
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Preparation of blood smear 1 Thin Blood Film Thin PBF can be prepared from anticoagulated blood obtained by venipuncture or from free-flowing finger-prick blood by any of the following three techniques. Common cause of a poor blood smear. Use clean high-quality microscope slides. This drop of blood is then placed on a clean glass slide about 1 cm from one end in the center line of the slide. Blood can also be taken from an EDTA sample. Select the finger to puncture usually the middle or ring finger.
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Always start with room temperature well-mixed clot free EDTA anticoagulated blood heparin is used for some exotic species. This PULLS the blood across to make the smear. Common cause of a poor blood smear. How to Make a Peripheral Blood Smear. Otherwise your smear may run right off of your horizontal slide and you will not have a nice feathered edge and your smear will be so thin you will have trouble finding enough cells.
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The scratches allow for improved adherence of the blood film to the slide without affecting the smear morphology. Common cause of a poor blood smear. Push carefully along the length of the first slide to produce a thin smear of blood. Take one drop of venous or capillary blood. All parts of the smear should be examined however the monolayer is the area where the cells are examined in close detail and differential cell.
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The drop and it spreads by capillary action along its edge. Collect blood in an EDTA tube and make the smears when back at the clinic. Components of a blood smear. A well made blood smear consists of several areas. Place either the long or the short edge of the pusher slide just in front of the blood drop holding it at an approximately 30-degree angle from the patient slide.
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The process is similar to making a normal thick film but instead of using a stick to spread the blood the edge of a glass microscope slide is used while applying firm pressure to create small scratches in the underlying slide. Preparation of blood smear 1 Thin Blood Film Thin PBF can be prepared from anticoagulated blood obtained by venipuncture or from free-flowing finger-prick blood by any of the following three techniques. Common cause of a poor blood smear. Blood Smear Technique 1 On one slide the sample slide place a small drop of blood 2 Use the second slide as a spreader slide. Always start with room temperature well-mixed clot free EDTA anticoagulated blood heparin is used for some exotic species.
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Collect blood in an EDTA tube and make the smears when back at the clinic. The process is similar to making a normal thick film but instead of using a stick to spread the blood the edge of a glass microscope slide is used while applying firm pressure to create small scratches in the underlying slide. Always start with room temperature well-mixed clot free EDTA anticoagulated blood heparin is used for some exotic species. Preparation of blood smear 1 Thin Blood Film Thin PBF can be prepared from anticoagulated blood obtained by venipuncture or from free-flowing finger-prick blood by any of the following three techniques. Place a small drop of blood or one side about 1-2 cm from one end.
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Do not push the blood by having it ahead of the smearing slide. Drop of blood too large or too small 2. 3 Place the end of the spreader slide on the sample slide so that the short sided edge of the spreader is below the drop of blood. Clean the area to be punctured with 70 alcohol. Place a drop of blood on the patient slide near the frosted edge.
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In this video I quickly go over how to make a peripheral blood smear. The slide should be clean. In this video I quickly go over how to make a peripheral blood smear. Proper preparation of the blood smear is critical for correct interpretation and obtaining accurate results. The blood smear is a simple blood test.
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Proper preparation of the blood smear is critical for correct interpretation and obtaining accurate results. 3 Place the end of the spreader slide on the sample slide so that the short sided edge of the spreader is below the drop of blood. Aim for a blood droplet size of 4mm diameter. The portion of blood film near the drop of blood. A well made blood smear consists of several areas.
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A quick instructional video demonstrating. Push the edge of the pusher slide into the blood drop so that it spreads out completely along the pusher slide. Can occur with anemic patients Increase the angle of the slide you are using to smear with. Making a quality blood smear Although there are several techniques described for making blood smears most people use the wedge or push technique. Place either the long or the short edge of the pusher slide just in front of the blood drop holding it at an approximately 30-degree angle from the patient slide.
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Preparation of blood smear 1 Thin Blood Film Thin PBF can be prepared from anticoagulated blood obtained by venipuncture or from free-flowing finger-prick blood by any of the following three techniques. Select the finger to puncture usually the middle or ring finger. Drop of blood too large or too small 2. Preparation of blood smear 1 Thin Blood Film Thin PBF can be prepared from anticoagulated blood obtained by venipuncture or from free-flowing finger-prick blood by any of the following three techniques. Preparation of Blood Film.
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Now push the spreader across the slide. Do not touch the surface of the slide where the blood smear will be made. This drop of blood is then placed on a clean glass slide about 1 cm from one end in the center line of the slide. Making a quality blood smear Although there are several techniques described for making blood smears most people use the wedge or push technique. The tapering end of the blood film.
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Clean slides with 70 to 90 alcohol and allow to dry. The process is similar to making a normal thick film but instead of using a stick to spread the blood the edge of a glass microscope slide is used while applying firm pressure to create small scratches in the underlying slide. Place either the long or the short edge of the pusher slide just in front of the blood drop holding it at an approximately 30-degree angle from the patient slide. Take one drop of venous or capillary blood. Parts of Blood Smear A peripheral blood film consists of 3 parts.
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All parts of the smear should be examined however the monolayer is the area where the cells are examined in close detail and differential cell. To make a short smear hold the spreader at a steeper angle and to make a longer smear hold it closer to the drop. A well made blood smear consists of several areas. Select the finger to puncture usually the middle or ring finger. This drop of blood is then placed on a clean glass slide about 1 cm from one end in the center line of the slide.
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Parts of Blood Smear A peripheral blood film consists of 3 parts. Select the finger to puncture usually the middle or ring finger. Blood Smear Technique 1 On one slide the sample slide place a small drop of blood 2 Use the second slide as a spreader slide. 3 Place the end of the spreader slide on the sample slide so that the short sided edge of the spreader is below the drop of blood. Take another clean slide and holding at an angle of about 45 deg touch the blood with one end of the slide so the blood runs along the edge of the slide by capillary action.
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Prick the ring finger of left hand with the help of prickling needle under all aseptic conditions discard the first two drOps of blood from skin puncture site. In this video I quickly go over how to make a peripheral blood smear. This PULLS the blood across to make the smear. All parts of the smear should be examined however the monolayer is the area where the cells are examined in close detail and differential cell. Can occur with anemic patients Increase the angle of the slide you are using to smear with.
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Drop of blood too large or too small 2. The portion of blood film near the drop of blood. How to Make a Peripheral Blood Smear. The drop and it spreads by capillary action along its edge. Preparation of blood smear 1 Thin Blood Film Thin PBF can be prepared from anticoagulated blood obtained by venipuncture or from free-flowing finger-prick blood by any of the following three techniques.
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A quick instructional video demonstrating. Components of a blood smear. Select the finger to puncture usually the middle or ring finger. Proper preparation of the blood smear is critical for correct interpretation and obtaining accurate results. A well made blood smear consists of several areas.
Source: pinterest.com
Blood Smear Technique 1 On one slide the sample slide place a small drop of blood 2 Use the second slide as a spreader slide. Proper preparation of the blood smear is critical for correct interpretation and obtaining accurate results. Do not push the blood by having it ahead of the smearing slide. Use clean high-quality microscope slides. Components of a blood smear.
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